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KMID : 1094720000050030202
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 3 p.202 ~ p.206
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Enhancement in the viability and biosensing activity of freeze-dried recombinant bioluminescent bacteria
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Choi Sue-Hyung
Gu Man-Bock
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Abstract
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The genetically-engineeredEscherichia coli strain, DPD2540, which contains afabA::luxCDABE fusion gene, gives a bioluminescent output when membrane fatty acid synthesis is needed. For more practical application of this strain in the field as biosensor, freeze-drying was adopted. A 12% sucrose solution with Luria-Bertani (LB) broth, as determined by the viability after freeze-drying, was found to be the most effective composition for lyophilization solution among various compositions tested. Rapid freezing with liquid nitrogen also gave the best viability after freeze-drying as compared to samples frozen at ?70°C and ?20°C. The biosensing activities of the cells showed a greater sensitivity when the cells from the exponential phase were freeze-dried. Finally, the optimum temperature for use of the freeze-dried cells in the biosensor field was determined.
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KEYWORD
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recombinant bioluminescent bacteria, bioluminescence, cryoprotectant, optimum freezing and monitoring temperatures
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